Purified Rabbit anti-TLE1 Antibody

SKU: DL96091A

Size 1: 100 ul

Synonyms : Transducin-like enhancer protein 1; E(Sp1) homolog; Enhancer of split groucho-like protein 1; ESG1

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to TLE1

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human TLE1

WB description: Western blot analysis of TLE1 expression in MCF7 (A), HT29 (B), mouse liver (C), mouse brain (D) whole cell lysates.

IHC description: Immunohistochemical analysis of TLE1 staining in mouse brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of TLE1 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)

Gene ID(Human): 7088

Gene ID (Mouse): 21885

Gene ID(Rat):

SwissProt (Human): Q04724

SwissProt (Mouse): Q62440

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.