Purified Rabbit anti-SPHK2 (pT614) Antibody

SKU: DL93832A

Size 1: 100 ul

Synonyms : Sphingosine kinase 2; SK 2; SPK 2

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M

Applications: WB, IHC

Background: Rabbit polyclonal antibody to SPHK2 (pT614)

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human SPHK2. The exact sequence is proprietary.

WB description: Western blot analysis of SPHK2 (pT614) expression in HEK293T (A), HepG2 (B) whole cell lysates.

IHC description: Immunohistochemical analysis of SPHK2 (pT614) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200)

Gene ID(Human): 56848

Gene ID (Mouse): 56632

Gene ID(Rat):

SwissProt (Human): Q9NRA0

SwissProt (Mouse): Q9JIA7

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.