Purified Rabbit anti-SPARC Antibody

SKU: DL92016A

Size 1: 100 ul

Synonyms : ON; SPARC; Basement-membrane protein 40; BM-40; Osteonectin; ON; Secreted protein acidic and rich in cysteine

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, B, S, Z

Applications: WB, IHC

Background: Rabbit polyclonal antibody to SPARC

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human SPARC. The exact sequence is proprietary.

WB description: Western blot analysis of SPARC expression in A549 (A), NIH3T3 (B), PC12 (C) whole cell lysates.

IHC description: Immunohistochemical analysis of SPARC staining in mouse muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200)

Gene ID(Human): 6678

Gene ID (Mouse): 20692

Gene ID(Rat): 24791

SwissProt (Human): P09486

SwissProt (Mouse): P07214

SwissProt (Rat): P16975

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.