Purified Rabbit anti-Smooth Muscle Actin Antibody

SKU: DL92750A

Size 1: 100 ul

Synonyms : ACTSA; ACTVS; Actin, aortic smooth muscle; Alpha-actin-2; Cell growth-inhibiting gene 46 protein

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, B, C, Rb

Applications: WB, IHC

Background: Rabbit polyclonal antibody to Smooth Muscle Actin

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Smooth Muscle Actin. The exact sequence is proprietary.

WB description: Western blot analysis of Smooth Muscle Actin expression in MCF7 (A), K562 (B) whole cell lysates.

IHC description: Immunohistochemical analysis of Smooth Muscle Actin staining in human skeletal muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200)

Gene ID(Human): 59

Gene ID (Mouse): 11475

Gene ID(Rat): 81633

SwissProt (Human): P62736

SwissProt (Mouse): P62737

SwissProt (Rat): P62738

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.