Purified Rabbit anti-SMAD2 Antibody

SKU: DL94712A

Size 1: 100 ul

Synonyms : MADH2; MADR2; Mothers against decapentaplegic homolog 2; MAD homolog 2; Mothers against DPP homolog 2; JV18-1; Mad-related protein 2; hMAD-2; SMAD family member 2; SMAD 2; Smad2; hSMAD2

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, B

Applications: WB, IHC

Background: Rabbit polyclonal antibody to SMAD2

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human SMAD2. The exact sequence is proprietary.

WB description: Western blot analysis of SMAD2 expression in COLO205 (A) whole cell lysates.

IHC description: Immunohistochemical analysis of SMAD2 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100)

Gene ID(Human): 4087

Gene ID (Mouse): 17126

Gene ID(Rat): 29357

SwissProt (Human): Q15796

SwissProt (Mouse): Q62432

SwissProt (Rat): O70436

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.