Purified Rabbit anti-SBDS Antibody

SKU: DL97985A

Size 1: 100 ul

Synonyms : Ribosome maturation protein SBDS; Shwachman-Bodian-Diamond syndrome protein

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to SBDS

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human SBDS

WB description: Western blot analysis of SBDS expression in HL60 (A), SHSY5Y (B) whole cell lysates.

IHC description: Immunohistochemical analysis of SBDS staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of SBDS staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)

Gene ID(Human): 51119

Gene ID (Mouse): 66711

Gene ID(Rat): 288615

SwissProt (Human): Q9Y3A5

SwissProt (Mouse): P70122

SwissProt (Rat): Q5RK30

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.