Purified Rabbit anti-RPL14 Antibody

SKU: DL97072A

Size 1: 100 ul

Synonyms : 60S ribosomal protein L14; CAG-ISL 7

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, R

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to RPL14

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human RPL14

WB description: Western blot analysis of RPL14 expression in Jurkat (A), HepG2 (B), mouse spleen (C), mouse ovary (D), rat kidney (E) whole cell lysates.

IHC description: Immunohistochemical analysis of RPL14 staining in rat liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of RPL14 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)

Gene ID(Human): 9045

Gene ID (Mouse):

Gene ID(Rat):

SwissProt (Human): P50914

SwissProt (Mouse):

SwissProt (Rat): Q63507

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.