Purified Rabbit anti-POLR3B Antibody

SKU: DL94206A

Size 1: 100 ul

Synonyms : DNA-directed RNA polymerase III subunit RPC2; RNA polymerase III subunit C2; C128; DNA-directed RNA polymerase III 127.6 kDa polypeptide; DNA-directed RNA polymerase III subunit B

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, B, D, Mk, P, Rb, Z

Applications: WB, IHC

Background: Rabbit polyclonal antibody to POLR3B

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human POLR3B. The exact sequence is proprietary.

WB description: Western blot analysis of POLR3B expression in Hela (A), HepG2 (B) whole cell lysates.

IHC description: Immunohistochemical analysis of POLR3B staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200)

Gene ID(Human): 55703

Gene ID (Mouse): 70428

Gene ID(Rat):

SwissProt (Human): Q9NW08

SwissProt (Mouse): P59470

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.