Purified Rabbit anti-PKC theta (pT538) Antibody

SKU: DL99232A

Size 1: 100 ul

Synonyms : PRKCT; Protein kinase C theta type; nPKC-theta

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IH

Background: Rabbit polyclonal antibody to PKC theta (pT538)

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PKC theta with a phosphorylation site at T538. The exact sequence is proprietary.

WB description: Western blot analysis of PKC theta (pT538) expression in mouse muscle (A), rat muscle (B) whole cell lysates.

IHC description: Immunohistochemical analysis of PKC theta (pT538) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200)

Gene ID(Human): 5588

Gene ID (Mouse): 18761

Gene ID(Rat):

SwissProt (Human): Q04759

SwissProt (Mouse): Q02111

SwissProt (Rat): Q9WTQ0

Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.