Purified Rabbit anti-PAK1 (pS199) Antibody

SKU: DL94756A

Size 1: 100 ul

Synonyms : Serine/threonine-protein kinase PAK 1; Alpha-PAK; p21-activated kinase 1; PAK-1; p65-PAK

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, B

Applications: WB, IHC

Background: Rabbit polyclonal antibody to PAK1 (pS199)

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PAK1. The exact sequence is proprietary.

WB description: Western blot analysis of PAK1 (pS199) expression in mouse brain (A) whole cell lysates.

IHC description: Immunohistochemical analysis of PAK1 (pS199) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100)

Gene ID(Human): 5058

Gene ID (Mouse):

Gene ID(Rat): 29431

SwissProt (Human): Q13153

SwissProt (Mouse): O88643

SwissProt (Rat): P35465

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.