Purified Rabbit anti-p53 (pS376) Antibody

SKU: DL94309A

Size 1: 100 ul

Synonyms : P53; Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M

Applications: WB, IHC

Background: Rabbit polyclonal antibody to p53 (pS376)

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human p53. The exact sequence is proprietary.

WB description: Western blot analysis of p53 (pS376) expression in NIH3T3 LPS-treated (A) whole cell lysates.

IHC description: Immunohistochemical analysis of p53 (pS376) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200)

Gene ID(Human): 7157

Gene ID (Mouse): 22059

Gene ID(Rat):

SwissProt (Human): P04637

SwissProt (Mouse): P02340

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.