Purified Rabbit anti-LIM Antibody

SKU: DL97006A

Size 1: 100 ul

Synonyms : ENH; PDZ and LIM domain protein 5; Enigma homolog; Enigma-like PDZ and LIM domains protein

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to LIM

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human LIM

WB description: Western blot analysis of LIM expression in HepG2 (A), MCF7 (B), mouse lung (C), mouse kidney (D) whole cell lysates.

IHC description: Immunohistochemical analysis of LIM staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of LIM staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)

Gene ID(Human): 10611

Gene ID (Mouse): 56376

Gene ID(Rat): 64353

SwissProt (Human): Q96HC4

SwissProt (Mouse): Q8CI51

SwissProt (Rat): Q62920

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.