Purified Rabbit anti-KDELR2 Antibody

SKU: DL93447A

Size 1: 100 ul

Synonyms : ERD2.2; ER lumen protein retaining receptor 2; ERD2-like protein 1; ELP-1; KDEL endoplasmic reticulum protein retention receptor 2; KDEL receptor 2

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, B, C, P, S, Z

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to KDELR2

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human KDELR2. The exact sequence is proprietary.

WB description: Western blot analysis of KDELR2 expression in SHSY5Y (A), NIH3T3 (B) whole cell lysates.

IHC description: Immunohistochemical analysis of KDELR2 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of KDELR2 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)

Gene ID(Human): 11014

Gene ID (Mouse): 66913

Gene ID(Rat): 304290

SwissProt (Human): P33947

SwissProt (Mouse): Q9CQM2

SwissProt (Rat): Q5U305

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.