Purified Rabbit anti-IFRD1 Antibody

SKU: DL98199A

Size 1: 100 ul

Synonyms : Interferon-related developmental regulator 1; Nerve growth factor-inducible protein PC4

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to IFRD1

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human IFRD1

WB description: Western blot analysis of IFRD1 expression in Hela (A), Raji (B), mouse lung (C), rat brain (D) whole cell lysates.

IHC description: Immunohistochemical analysis of IFRD1 staining in human lung formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of IFRD1 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)

Gene ID(Human): 3475

Gene ID (Mouse): 15982

Gene ID(Rat): 29596

SwissProt (Human): O00458

SwissProt (Mouse): P19182

SwissProt (Rat): P20695

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.