Purified Rabbit anti-HNF4 alpha/gamma Antibody

SKU: DL94871A

Size 1: 100 ul

Synonyms : HNF4A; HNF4; NR2A1; TCF14; Hepatocyte nuclear factor 4-alpha; HNF-4-alpha; Nuclear receptor subfamily 2 group A member 1; Transcription factor 14; TCF-14; Transcription factor HNF-4; HNF4G; NR2A2; Hepatocyte nuclear factor 4-gamma; HNF-4-gamma; Nuclear receptor subfamily 2 group A member 2

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to HNF4 alpha/gamma

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human HNF4 alpha/gamma. The exact sequence is proprietary.

WB description: Western blot analysis of HNF4 alpha/gamma expression in A549 (A), HepG2 (B), Hela (C) whole cell lysates.

IHC description: Immunohistochemical analysis of HNF4 alpha/gamma staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of HNF4 alpha/gamma staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)

Gene ID(Human): 3172; 3174

Gene ID (Mouse): 15378; 30942

Gene ID(Rat): 25735

SwissProt (Human): P41235; Q14541

SwissProt (Mouse): P49698; Q9WUU6

SwissProt (Rat): P22449

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.