Purified Rabbit anti-Histone Deacetylase 6 Antibody

SKU: DL92991A

Size 1: 100 ul

Synonyms : KIAA0901; Histone deacetylase 6; HD6

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, Mk

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to Histone Deacetylase 6

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Histone Deacetylase 6. The exact sequence is proprietary.

WB description: Western blot analysis of Histone Deacetylase 6 expression in PC3 (A), Jurkat (B), NIH3T3 (C), rat liver (D) whole cell lysates.

IHC description: Immunohistochemical analysis of Histone Deacetylase 6 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of Histone Deacetylase 6 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)

Gene ID(Human): 10013

Gene ID (Mouse): 15185

Gene ID(Rat):

SwissProt (Human): Q9UBN7

SwissProt (Mouse): Q9Z2V5

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.