Purified Rabbit anti-Histone Deacetylase 10 Antibody

SKU: DL91807A

Size 1: 100 ul

Synonyms : Histone deacetylase 10; HD10

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, Mk

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to Histone Deacetylase 10

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Histone Deacetylase 10. The exact sequence is proprietary.

WB description: Western blot analysis of Histone Deacetylase 10 expression in HeLa (A), Jurkat (B), mouse liver (C), rat liver (D) whole cell lysates.

IHC description: Immunohistochemical analysis of Histone Deacetylase 10 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of Histone Deacetylase 10 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)

Gene ID(Human): 83933

Gene ID (Mouse): 170787

Gene ID(Rat): 362981

SwissProt (Human): Q969S8

SwissProt (Mouse): Q6P3E7

SwissProt (Rat): Q569C4

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.