Purified Rabbit anti-Hexokinase 1 Antibody

SKU: DL93005A

Size 1: 100 ul

Synonyms : Hexokinase-1; Brain form hexokinase; Hexokinase type I; HK I

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, B, C, D, P, Z

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to Hexokinase 1

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Hexokinase 1. The exact sequence is proprietary.

WB description: Western blot analysis of Hexokinase 1 expression in HEK293T (A), mouse brain (B) whole cell lysates.

IHC description: Immunohistochemical analysis of Hexokinase 1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of Hexokinase 1 staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)

Gene ID(Human): 3098

Gene ID (Mouse): 15275

Gene ID(Rat): 25058

SwissProt (Human): P19367

SwissProt (Mouse): P17710

SwissProt (Rat): P05708

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.