Purified Rabbit anti-HAGH Antibody

SKU: DL96290A

Size 1: 100 ul

Synonyms : GLO2; HAGH1; Hydroxyacylglutathione hydrolase, mitochondrial; Glyoxalase II; Glx II

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to HAGH

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human HAGH

WB description: Western blot analysis of HAGH expression in HepG2 (A), mouse liver (B), mouse heart (C), rat kidney (D) whole cell lysates.

IHC description: Immunohistochemical analysis of HAGH staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of HAGH staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/10 - 1/100)

Gene ID(Human): 3029

Gene ID (Mouse):

Gene ID(Rat):

SwissProt (Human): Q16775

SwissProt (Mouse):

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.