Purified Rabbit anti-Focal Adhesion Kinase Antibody

SKU: DL98642A

Size 1: 100 ul

Synonyms : FAK; FAK1; Focal adhesion kinase 1; FADK 1; Focal adhesion kinase-related nonkinase; FRNK; Protein phosphatase 1 regulatory subunit 71; PPP1R71; Protein-tyrosine kinase 2; p125FAK; pp125FAK

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IH

Background: Rabbit polyclonal antibody to Focal Adhesion Kinase

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Focal Adhesion Kinase. The exact sequence is proprietary.

WB description: Western blot analysis of Focal Adhesion Kinase expression in SKOVCAR3 (A), LOVO (B), Beas2B (C), A549 (D), PC3 (E), NIH3T3 (F), CT26 (G) whole cell lysates.

IHC description: Immunohistochemical analysis of Focal Adhesion Kinase staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200)

Gene ID(Human): 5747

Gene ID (Mouse): 14083

Gene ID(Rat): 25614

SwissProt (Human): Q05397

SwissProt (Mouse): P34152

SwissProt (Rat): O35346

Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.