Purified Rabbit anti-DDX1 Antibody

SKU: DL96275A

Size 1: 100 ul

Synonyms : ATP-dependent RNA helicase DDX1; DEAD box protein 1; DEAD box protein retinoblastoma; DBP-RB

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to DDX1

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human DDX1

WB description: Western blot analysis of DDX1 expression in MCF7 (A), HepG2 (B), mouse brain (C), rat brain (D) whole cell lysates.

IHC description: Immunohistochemical analysis of DDX1 staining in mouse kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of DDX1 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)

Gene ID(Human): 1653

Gene ID (Mouse): 104721

Gene ID(Rat): 84474

SwissProt (Human): Q92499

SwissProt (Mouse): Q91VR5

SwissProt (Rat): Q641Y8

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.