Purified Rabbit anti-cTnI Antibody

SKU: DL93288A

Size 1: 100 ul

Synonyms : TNNI3; TNNC1; Troponin I, cardiac muscle; Cardiac troponin I

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: M, R, P, Rb

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to cTnI

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human cTnI. The exact sequence is proprietary.

WB description: Western blot analysis of over-expressed FLAG-tagged protein in 293T cell lysate.

IHC description:

IF/ICC description: Immunofluorescent analysis of FLAG-tag staining in 293T cells transfected with a Flag-tag protein. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

IP description: Immunoprecipitation of FLAG-tagged protein from HEK293T cells transfected with vector overexpressing Flag tag, using anti-FLAG-tag Antibody.

FC description:

ChIP description:

Purification_Method:

Clonality: Monoclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/2000 - 1/5000), IF/IC (1/200 - 1/500), IP (1/100 - 1/200)

Gene ID(Human):

Gene ID (Mouse):

Gene ID(Rat):

SwissProt (Human):

SwissProt (Mouse):

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.