Purified Rabbit anti-Collagen 4 alpha 1 Antibody

SKU: DL90254A

Size 1: 100 ul

Synonyms : Collagen alpha-1(IV) chain

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, B, C, P

Applications: WB, IHC

Background: Rabbit polyclonal antibody to Collagen 4 alpha 1

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Collagen 4 alpha 1. The exact sequence is proprietary.

WB description: Western blot analysis of Collagen 4 alpha 1 expression in HEK293T (A), mouse endothelial (B) whole cell lysates.

IHC description: Immunohistochemical analysis of Collagen 4 alpha 1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200)

Gene ID(Human): 1282

Gene ID (Mouse): 12826

Gene ID(Rat):

SwissProt (Human): P02462

SwissProt (Mouse): P02463

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.