Purified Rabbit anti-Collagen 11 alpha 1 Antibody

SKU: DL90258A

Size 1: 100 ul

Synonyms : COLL6; Collagen alpha-1(XI) chain

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, B

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to Collagen 11 alpha 1

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Collagen 11 alpha 1. The exact sequence is proprietary.

WB description: Western blot analysis of Collagen 11 alpha 1 expression in K562 (A), Saos2 (B) whole cell lysates.

IHC description: Immunohistochemical analysis of Collagen 11 alpha 1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of Collagen 11 alpha 1 staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)

Gene ID(Human): 1301

Gene ID (Mouse): 12814

Gene ID(Rat): 25654

SwissProt (Human): P12107

SwissProt (Mouse): Q61245

SwissProt (Rat): P20909

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.