Purified Rabbit anti-Claudin 5 Antibody

SKU: DL91173A

Size 1: 100 ul

Synonyms : AWAL; TMVCF; Claudin-5; Transmembrane protein deleted in VCFS; TMDVCF

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, B, P, Rb

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to Claudin 5

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Claudin 5. The exact sequence is proprietary.

WB description: Western blot analysis of Claudin 5 expression in HeLa (A), mouse liver (B), rat lung (C) whole cell lysates.

IHC description: Immunohistochemical analysis of Claudin 5 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of Claudin 5 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)

Gene ID(Human): 7122

Gene ID (Mouse): 12741

Gene ID(Rat): 65131

SwissProt (Human): O00501

SwissProt (Mouse): O54942

SwissProt (Rat): Q9JKD6

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.