Purified Rabbit anti-CD66f Antibody

SKU: DL97049A

Size 1: 100 ul

Synonyms : B1G1; PSBG1; PSGGA; Pregnancy-specific beta-1-glycoprotein 1; PS-beta-G-1; PSBG-1; Pregnancy-specific glycoprotein 1; CD66 antigen-like family member F; Fetal liver non-specific cross-reactive antigen 1/2; FL-NCA-1/2; PSG95; Pregnancy-specific beta-1 glycoprotein C/D; PS-beta-C/D; CD66f

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to CD66f

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human CD66f

WB description: Western blot analysis of CD66f expression in SW480 (A), A549 (B), Hela (C) whole cell lysates.

IHC description: Immunohistochemical analysis of CD66f staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of CD66f staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/100)

Gene ID(Human): 5669

Gene ID (Mouse):

Gene ID(Rat):

SwissProt (Human): P11464

SwissProt (Mouse):

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.