Purified Rabbit anti-Caspase 3 p17 Antibody

SKU: DL90141A

Size 1: 100 ul

Synonyms : CPP32; Caspase-3; CASP-3; Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1; SCA-1

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, B, Mk, S

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to Caspase 3 p17

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Caspase 3 p17. The exact sequence is proprietary.

WB description: Western blot analysis of Caspase 3 p17 expression in SP2/0 (A), Jurkat etoposide-treated (B), NIH3T3 staurosporine-treated (C), HeLa (D) whole cell lysates.

IHC description: Immunohistochemical analysis of Caspase 3 p17 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of Caspase 3 p17 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)

Gene ID(Human): 836

Gene ID (Mouse): 12367

Gene ID(Rat): 25402

SwissProt (Human): P42574

SwissProt (Mouse): P70677

SwissProt (Rat): P55213

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.