Purified Rabbit anti-CaMK1 alpha (pT177) Antibody

SKU: DL92811A

Size 1: 100 ul

Synonyms : Calcium/calmodulin-dependent protein kinase type 1; CaM kinase I; CaM-KI; CaM kinase I alpha; CaMKI-alpha

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to CaMK1 alpha (pT177)

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CaMK1 alpha (pT177). The exact sequence is proprietary.

WB description: Western blot analysis of CaMK1 alpha (pT177) expression in Jurkat Insulin-treated (A) whole cell lysates.

IHC description: Immunohistochemical analysis of CaMK1 alpha (pT177) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of CaMK1 alpha (pT177) staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)

Gene ID(Human): 8536

Gene ID (Mouse): 52163

Gene ID(Rat): 171503

SwissProt (Human): Q14012

SwissProt (Mouse): Q91YS8

SwissProt (Rat): Q63450

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.