Purified Rabbit anti-c-RAF Antibody

SKU: DL95611A

Size 1: 100 ul

Synonyms : RAF; RAF proto-oncogene serine/threonine-protein kinase; Proto-oncogene c-RAF; cRaf; Raf-1

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H

Applications: WB, IHC

Background: Rabbit polyclonal antibody to c-RAF

Concentration: 1mg/mL

Immunogen: Recombinant protein corresponding to human c-RAF.

WB description: Western blot analysis of c-RAF expression in Hela (A) whole cell lysates.

IHC description: Immunohistochemical analysis of c-RAF staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/1000 - 1/2000), IH (1/50 - 1/200)

Gene ID(Human): 5894

Gene ID (Mouse):

Gene ID(Rat):

SwissProt (Human): P04049

SwissProt (Mouse):

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.