Purified Rabbit anti-BRAF35 Antibody

SKU: DL93007A

Size 1: 100 ul

Synonyms : BRAF35; HMGX2; HMGXB2; SMARCE1R; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1-related; SMARCE1-related protein; BRCA2-associated factor 35; HMG box-containing protein 20B; HMG domain-containing protein 2; HMG domain-containing protein HMGX2; Sox-like transcriptional factor; Structural DNA-binding protein BRAF35

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to BRAF35

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human BRAF35. The exact sequence is proprietary.

WB description: Western blot analysis of BRAF35 expression in HeLa (A), HEK293T (B) whole cell lysates.

IHC description: Immunohistochemical analysis of BRAF35 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of BRAF35 staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)

Gene ID(Human): 10362

Gene ID (Mouse):

Gene ID(Rat):

SwissProt (Human): Q9P0W2

SwissProt (Mouse):

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.