Purified Mouse anti-SMAD3 Antibody

SKU: DL95549A

Size 1: 100 ul

Synonyms : MADH3; Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3

host: Mouse

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC

Background: Mouse monoclonal antibody to SMAD3

Concentration: 1mg/mL

Immunogen: Recombinant protein corresponding to human SMAD3.

WB description: Western blot analysis of SMAD3 expression in 293T (A), Jurkat (B), mouse heart (C), rat heart (D) whole cell lysates.

IHC description: Immunohistochemical analysis of SMAD3 staining in human liver,human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method:

Clonality: Monoclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/1000 - 1/2000), IH (1/100 - 1/200)

Gene ID(Human): 4088

Gene ID (Mouse):

Gene ID(Rat):

SwissProt (Human): P84022

SwissProt (Mouse):

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.