GeneBio Systems
PS(Phosphatidylserine) ELISA Kit
PS(Phosphatidylserine) ELISA Kit
SKU:ELK8149
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Size:96T
Reactivity:General
Alternative Names:Ptd-L-Ser; PtdSer
Assay Type:Competitive Inhibition
Sensitivity:5.16 ng/mL
Standard:1200 ng/mL
Detection range:18.75-1200 ng/mL
Sample type:Serum, plasma and other biological fluids
Assay length:2h
Research Area:Neuro science;Developmental science;Nutrition metabolism;
Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with PS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PS in the samples is then determined by comparing the OD of the samples to the standard curve.
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