PROA(Protein A) ELISA Kit

SKU: ELK0772

Size:96T

Reactivity:General

Alternative Names:Staphylococcus aureus protein A (SpA)

Assay Type:Competitive Inhibition

Sensitivity:19.1 pg/mL

Standard:3000 pg/mL

Detection range:46.88-3000 pg/mL

Sample type:serum, plasma, other biological fluids

Assay length:2.5h

Research Area:Tumor immunity;Endocrinology;Cardiovascular biology;

Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with PROA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PROA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PROA in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: