PKM Antibody - Cat. #: CSB-PA984745

SKU: CSB-PA984745

Size :100ul

Clone Number:

Aliases:PKM; PK3; OIP3; PK2;

Product Type:Polyclonal Antibody

Immunogen Species:Homo sapiens (Human)

UniProt ID:P14618-1

Immunogen:Peptide sequence around aa.405~409(T-S-D-P-T) derived from Human PKM2.

Raised in:Rabbit

Species Reactivity:Human

Tested Applications:ELISA, WB, IF; WB:1:500-1:1000, IF:1:100-1:200

Background:

Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.

Christofk, H.R. et al. (2008) Nature 452, 230-3.
Mazurek, S. et al. (2005) Semin Cancer Biol 15, 300-8.
Dombrauckas, J.D. et al. (2005) Biochemistry 44, 9417-29.
Hitosugi, T. et al. (2009) Sci Signal 2, ra73.

Clonality:Polyclonal

Isotype:IgG

Purification Method:Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Conjugate:Non-conjugated

Buffer:Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form:liquid

Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names:PKM

Research Areas:Epigenetics and Nuclear Signaling;Cancer;Metabolism;Signal transduction