PKA I Alpha reg Polyclonal Antibody

SKU: BT-AP07201

Size:100μL

Background:cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. PRKAR1A encodes one of the regulatory subunits. Protein kinase cAMP-dependent type I regulatory subunit alpha was found to be a tissue-specific extinguisher that down-regulates the expression of seven liver genes in hepatoma x fibroblast hybrids. Mutations in PRKAR1A cause Carney complex (CNC). PRKAR1A can fuse to the RET protooncogene by gene rearrangement and form the thyroid tumor-specific chimeric oncogene known as PTC2. A nonconventional nuclear localization sequence (NLS) has been found for protein kinase cAMP-dependent type I regulatory subunit alpha which suggests a role in DNA replication via the protein serving as a nuclear transport protein for the second subunit of the Replication Factor C (RFC40). Several alternatively spliced transcript variants encoding two different isoforms have been observed.

Research_area:Signal Transduction

Target_protein:PRKAR1A

applications:WB, IHC-p, IF, ELISA

Reactivity:Human, Mouse, Rat

Clonality:Polyclonal

Clone ID:

Host:Rabbit

Isotype:IgG

Gene Symbol/ Name:PKA Ialpha reg Antibody

Immunogen:The antiserum was produced against synthesized peptide derived from human AMPK alpha around the phosphorylation site of Thr172. AA range:140-189

Storage:-20°C for 1 year

Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Formulation:Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration:1 mg/ml

Molecular weight(Da):42982

UniProt accession:Human: P10644; Mouse: Q9DBC7; Rat: P09456

Synonyms:PRKAR1A; PKR1; PRKAR1; TSE1; cAMP-dependent protein kinase type I-alpha regulatory subunit; Tissue-specific extinguisher 1; TSE1

GeneID:Human: 5573; Mouse: 19084; Rat: 25725