Pig MYO(Myoglobin) ELISA Kit

SKU: ELK10730

Size:96T

Reactivity:Pig

Alternative Names:PVALB; MB

Assay Type:Sandwich

Sensitivity:1.13 ng/mL

Standard:200 ng/mL

Detection range:3.13-200 ng/mL

Sample type:Serum, plasma, tissue homogenates and other biological fluids

Assay length:3.5h

Research Area:Cardiovascular biology;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig MYO. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig MYO. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig MYO, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig MYO in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:P02189