Phospho-Smad5 (Ser463/Ser465) Antibody , Cat. # AF3899

SKU: AF3899

Size:100ul

Host:Rabbit

Clonality:Polyclonal

Isotype:IgG

GeneName:SMAD5

GeneID:4090

Uniprot:Q99717

Specificity:Phospho-Smad5 (Ser463/Ser465) Antibody detects endogenous levels of Smad5 only when phosphorylated at Ser463/465.

Immunogen:A synthesized peptide derived from human Smad5 around the phosphorylation site of Ser463/465.

Storage:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Reactivity:Human,Mouse,Rat

Prediction:

Application:WB,IHC,ELISA(peptide)

Dilution:IHC 1:50-1:200, WB 1:500-1:2000, ELISA(peptide) 1:20000-1:40000

Conjugate:Unconjugated

RRID:AB_2847213

An Introduction to Blocking Peptides:A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site.

Cat# Blocking Peptide:AF3899-BP

Des# Blocking Peptide:Phospho-Smad5 (Ser463/Ser465) Blocking peptide

Blocking Peptide Price:$549 CAD