Phospho-RASGRF1 (Ser916) Antibody - Cat. #: CSB-PA256747

SKU: CSB-PA256747

Size :100ul

Clone Number:

Aliases:CDC25 antibody; CDC25L antibody; GNRP antibody; GRF1 antibody; GRF55 antibody; Guanine nucleotide exchange factor antibody; Guanine nucleotide releasing factor 55 kD antibody; Guanine nucleotide releasing protein antibody; guanine nucleotide-releasing factor 1 antibody; Guanine nucleotide-releasing protein antibody; H GRF55 antibody; HGRF55 antibody; PP13187 antibody; Ras protein specific guanine nucleotide releasing factor 1 antibody; Ras specific guanine nucleotide releasing factor antibody; Ras specific guanine nucleotide releasing factor CDC25 homolog antibody; Ras specific nucleotide exchange factor CDC25 antibody; Ras-GRF1 antibody; Ras-specific guanine nucleotide-releasing factor 1 antibody; Ras-specific nucleotide exchange factor CDC25 antibody; RASGRF1 antibody; RGRF1_HUMAN antibody

Product Type:Polyclonal Antibody

Immunogen Species:Homo sapiens (Human)

UniProt ID:Q13972

Immunogen:Peptide sequence around phosphorylation site of Serine 916(N-E-G(p)-T P) derived from Human Ras-GRF1.

Raised in:Rabbit

Species Reactivity:Mouse, Rat

Tested Applications:ELISA, IHC; IHC:1:50-1:100

Background:

Ras activity is regulated by GAP (GTPase activating proteins) and GEFs (guanine nucleotide exchange factors). Ras-GRF1 (also known as CDC25Mm) is neuronal RasGEF and is regulated by heterotrimeric G proteins and calcium influx . Binding to calmodulin and phosphorylation stimulate Ras-GRF1 activity. Multiple PKA phosphorylation sites on Ras-GRF have been identified. Phosphorylation on the two major sites, Ser54 and Ser822, inhibits Ras-GRF activity. Carbachol (a muscarinic agonist)-induced phosphorylation on Ser916 is essential but not sufficient for maximal Ras-GRF activity.

Huibin Yang, J. Biol. Chem., Apr 2003; 278: 13278 - 13285.
Huibin Yang, Mol. Biol. Cell, May 2006; 17: 2177 - 2189.
Raymond R. Mattingly, J. Biol. Chem., Dec 1999; 274: 37379.
Tie-Nian Zhu, J. Biol. Chem., May 2007; 282: 14816 - 14826.

Clonality:Polyclonal

Isotype:IgG

Purification Method:Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Conjugate:Non-conjugated

Buffer:Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form:liquid

Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names:RASGRF1

Research Areas:Signal transduction