Gene Bio Systems
Phospho-PRK1/2 (Thr774/Thr816) Antibody , Cat. # AF8349
Phospho-PRK1/2 (Thr774/Thr816) Antibody , Cat. # AF8349
SKU:AF8349
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Size:100ul
Host:Rabbit
Clonality:Polyclonal
Isotype:IgG
GeneName:PKN1,PKN2
GeneID:5585,5586
Uniprot:Q16512/Q16513
Specificity:Phospho-PRK1/2 (Thr774/Thr816) Antibody detects endogenous levels of PRK1/2 only when phosphorylated at Thr774/816.
Immunogen:A synthesized peptide derived from human PRK1/2 around the phosphorylation site of Thr774/816.
Storage:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Reactivity:Human,Rat
Prediction:Pig,Bovine,Sheep,Dog,Xenopus
Application:WB,IHC,ELISA(peptide)
Dilution:WB 1:1000-3000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000
Conjugate:Unconjugated
RRID:AB_2840411
An Introduction to Blocking Peptides:A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site.
Cat# Blocking Peptide:AF8349-BP
Des# Blocking Peptide:Phospho-PRK1/2 (Thr774/Thr816) Blocking peptide
Blocking Peptide Price:$549 CAD
