Phospho-pan-AKT1/2/3 (Tyr315/Tyr316/Tyr312) Antibody , Cat. # AF1022

SKU: AF1022

Size:100ul

Host:Rabbit

Clonality:Polyclonal

Isotype:IgG

GeneName:AKT1,AKT2,AKT3

GeneID:207,208,10000

Uniprot:P31749/P31751/Q9Y243

Specificity:Phospho-pan-AKT1/2/3 (Tyr315/Tyr316/Tyr312) Antibody detects endogenous levels of pan-AKT1/2/3 only when phosphorylated at Tyr315/316/312.

Immunogen:A synthesized peptide derived from human pan-AKT1/2/3 around the phosphorylation site of Tyr315/316/312.

Storage:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Reactivity:Human,Mouse,Rat

Prediction:

Application:WB,IHC,IF/ICC

Dilution:WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500

Conjugate:Unconjugated

RRID:AB_2845461

An Introduction to Blocking Peptides:A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site.

Cat# Blocking Peptide:AF1022-BP

Des# Blocking Peptide:Phospho-pan-AKT1/2/3 (Tyr315/Tyr316/Tyr312) Blocking peptide

Blocking Peptide Price:$549 CAD