Phospho-FOXO1/FOXO3/FOXO4 (Thr24/32) Antibody - Cat. #: CSB-PA196080

SKU: CSB-PA196080

Size :100ul

Clone Number:

Aliases:FKH 1 antibody; FKH1 antibody; FKHR antibody; Forkhead (Drosophila) homolog 1 (rhabdomyosarcoma) antibody; Forkhead box O1 antibody; Forkhead box protein O1 antibody; Forkhead box protein O1A antibody; Forkhead in rhabdomyosarcoma antibody; Forkhead; Drosophila; homolog of; in rhabdomyosarcoma antibody; FoxO transcription factor antibody; foxo1 antibody; FOXO1_HUMAN antibody; FOXO1A antibody; OTTHUMP00000018301 antibody

Product Type:Polyclonal Antibody

Immunogen Species:Homo sapiens (Human)

UniProt ID:Q12778

Immunogen:Peptide sequence around phosphorylation site of threonine 24/32(S-C-T(p)-W-P) derived from Human FOXO1/3/4-pan .

Raised in:Rabbit

Species Reactivity:Human, Mouse, Rat

Tested Applications:ELISA, WB; WB:1:500-1:1000

Background:

Transcription factor that is the main target of insulin signaling and regulates metabolic homeostasis in response to oxidative stress. Binds to the insulin response element (IRE) with consensus sequence 5'-TT[G/A]TTTTG-3' and the related Daf-16 family binding element (DBE) with consensus sequence 5'-TT[G/A]TTTAC-3'. Activity suppressed by insulin. Main regulator of redox balance and osteoblast numbers and controls bone mass. Orchestrates the endocrine function of the skeleton in regulating glucose metabolism. Acts syngernistically with ATF4 to suppress osteocalcin/BGLAP activity, increasing glucose levels and triggering glucose intolerance and insulin insensitivity. Also suppresses the transcriptional activity of RUNX2, an upstream activator of osteocalcin/BGLAP. In hepatocytes, promotes gluconeogenesis by acting together with PPARGC1A to activate the expression of genes such as IGFBP1, G6PC and PPCK1. Important regulator of cell death acting downstream of CDK1, PKB/AKT1 and SKT4/MST1. Promotes neural cell death. Mediates insulin action on adipose. Regulates the expression of adipogenic genes such as PPARG during preadipocyte differentiation and, adipocyte size and adipose tissue-specific gene expression in response to excessive calorie intake. Regulates the transcriptional activity of GADD45A and repair of nitric oxide-damaged DNA in beta-cells.

Robert J. Southgate, J. Biol. Chem., Jul 2007; 282: 21176 - 21186.
Susumu Kodama, Mol. Cell. Biol., Sep 2004; 24: 7931 - 7940.
Dragan Marinkovic, J. Clin. Invest., Aug 2007; 117: 2133 - 2144.
Marie-Liesse Asselin-Labat, Blood, Jul 2004; 104: 215 - 223.

Clonality:Polyclonal

Isotype:IgG

Purification Method:Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Conjugate:Non-conjugated

Buffer:Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form:liquid

Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names:FOXO1/FOXO3/FOXO4

Research Areas:Epigenetics and Nuclear Signaling?Neuroscience?Cancer;Metabolism;Signal transduction