Phospho-FGFR1/2 (Tyr463/Tyr466) Antibody , Cat. # AF3730

SKU: AF3730

Size:100ul

Host:Rabbit

Clonality:Polyclonal

Isotype:IgG

GeneName:FGFR1,FGFR2

GeneID:2260,2263

Uniprot:P11362/P21802

Specificity:Phospho-FGFR1/2 (Tyr463/Tyr466) Antibody detects endogenous levels of FGFR1/2 only when phosphorylated at Tyr463/466.

Immunogen:A synthesized peptide derived from human FGFR1/2 around the phosphorylation site of Tyr463/466.

Storage:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Reactivity:Human,Mouse,Rat

Prediction:

Application:IF/ICC,ELISA(peptide)

Dilution:IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Conjugate:Unconjugated

RRID:AB_2847044

An Introduction to Blocking Peptides:A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site.

Cat# Blocking Peptide:AF3730-BP

Des# Blocking Peptide:Phospho-FGFR1/2 (Tyr463/Tyr466) Blocking peptide

Blocking Peptide Price:$549 CAD