NFkB p65 Mouse mAb

SKU: EM1111

Size: 100μL

Source:Mouse

Applications:WB, IHC, IF, IP

Reactivity:Human, Rat, Mouse

Dilution:WB 1:500-2,000 IP 1:200 IF 1:100-200 IHC 1:200-500

Immunogen:Recombinant Protein

Storage_stability:PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Clonality:Monoclonal

Isotype:IgG1

Concentration:1mg/mL

Observed_band(KD):65kDa

Human_gene_id:5970

Human_swiss_prot_no:Q04206

Subcellular_location:Cytoplasm, Nucleus

Other_name:NFKB3, p65, RELA, Transcription factor p65

Background:NFkB p65 is ubiquitinated leading to its proteosomal degradation, which is required for termination of the NFkB response. Phosphorylation of NFkB p65 on S536 stimulates acetylation of K310 by CBP, enhancing transcriptional activity. NFkB p65 is also acetylated at K122, enhancing DNA binding and impairing the interaction with NFKBIA. The protein is deacetylated by HDAC3. Invasion of a host by a pathogen is frequently associated with the activation of NF-kB, which coordinates various aspects of immune function required for resistance to infection.