MUC5AC ELISA kit (Human)

SKU: SEA756Hu

Size: 96Tests

# of Times Cited in literature: 30

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: MUC5AC

Target Full Name: Mucin 5 Subtype AC

Alternative Names: TBM; MUC5; LeB; Oligomeric Mucus/Gel-Forming; Mucin 5,Subtypes A And C,Tracheobronchial/Gastric; Gastric mucin; Lewis B blood group antigen; Major airway glycoprotein

Target Species: Human

Uniprot: P98088

Gene ID: 4586

Featured Series: SE kit

Featured Series Function: Detects protein (regular version)

Specificity: Reactive with Human MUC5AC / Mucin 5 Subtype AC

Method: Colormetric

Detection principle: Double-antibody Sandwich

Detection range: 78-5,000pg/mL

Sensitivity: 30pg/mL

Assay Time: 3h

Sample Size: 100uL

Recommended/Predicted Sample Types: Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mucin 5 Subtype AC (MUC5AC) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mucin 5 Subtype AC (MUC5AC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Mucin 5 Subtype AC (MUC5AC). No significant cross-reactivity or interference between Mucin 5 Subtype AC (MUC5AC) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C; 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 3 times; 5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 6. Aspirate and wash 5 times; 7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 8. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mucin 5 Subtype AC (MUC5AC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mucin 5 Subtype AC (MUC5AC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mucin 5 Subtype AC (MUC5AC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mucin 5 Subtype AC (MUC5AC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Research Area: Tumor immunity;Infection immunity;Endocrinology;Pulmonology;

References Citing This Product: Pancreatic cyst fluid and serum mucin levels predict dysplasia in intraductal papillary mucinous neoplasms of the pancreas.

Naringin attenuates EGF-induced MUC5AC secretion in A549 cells by suppressing the cooperative activities of MAPKs-AP-1 and IKKs-IκB-NF-κB signaling pathways

Pyocyanin-induced mucin production is associated with redox modification of FOXA2

A novel serum marker for biliary tract cancer: Diagnostic and prognostic values of quantitative evaluation of serum mucin 5AC (MUC5AC)

The human cathelicidin LL-37 enhances airway mucus production in chronic obstructive pulmonary disease

Proteomic analysis of cancer and mesothelial cells reveals an increase in Mucin 5AC during ovarian cancer and peritoneal interaction

The human Cathelicidin LL-37 induces MUC5AC mucin production by airway epithelial cells via TACE-TGF-α-EGFR pathway

Assessment of bile and serum mucin5AC in cholangiocarcinoma: Diagnostic performance and biologic significance

Leukotriene E 4 induces MUC5AC release from human airway epithelial NCI-H292 cells

Secreted mucins and airway bacterial colonization in non-CF bronchiectasis

Mucin Secretion In Asthmatics With Osmotic Airway Hyperresponsiveness

ReCavia (Guinea pig )lation of Viral Infection-induced Airway Remodeling Cytokine Production by the TLR3-EGFR Signaling Pathway in Human Bronchial Epithelial Cells

The influence of long-term corneal contact lens wearing on the stability and quality of tear film in Qinghai

Airway Mucin 2 Is Decreased in Patients with Severe Chronic Obstructive Pulmonary Disease with Bacterial Colonization

Impact of Cigarette Smoking on Tear Function and Correlation between Conjunctival Goblet Cells and Tear MUC5AC Concentration in Office Workers

Interleukin-33 induces mucin gene expression and goblet cell hyperplasia in human nasalepithelial cells.

Clarithromycin inhibits TNF-α-induced MUC5AC mucin gene expression via