Mouse Hepatic lipase (HL) ELISA Kit

SKU: AE35471MO

Size: 96T. Other sizes are also available.

Species Reactivity: Mouse (Mus musculus)

UniProt: P27656

Abbreviation: LIPC

Alternative Names: HDLCQ12; HL; HTGL; LIPH; lipase C

Application: ELISA

Range: 0.312-20 ng/mL

Sensitivity: 0.126 ng/mL

Test principle: This assay employs a two-site sandwich ELISA to quantitate LIPC in samples. An antibody specific for LIPC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLIPC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LIPC is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LIPC bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: Hepatic lipase is a form of lipase. It is expressed in the liver and adrenal glands.One of the principal functions of hepatic lipase is to convert IDL to LDL.LIPC encodes hepatic triglyceride lipase, which is expressed in liver. LIPC has the dual functions of triglyceride hydrolase and ligand/bridging factor for receptor-mediated lipoprotein uptake. Hepatic lipase deficiency is a rare, autosomal recessive disorder that results in elevated high density lipoprotein (HDL) cholesterol due to a mutation in the hepatic lipase gene. Clinical features are not well understood and there are no characteristic xanthomas. There is an association with a delay in atherosclerosis in an animal model.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).