Mouse DNA (cytosine-5)-methyltransferase 3-like,DNMT3L ELISA KIT

SKU: E1492Mo

Size:96 Tests-1 Kit

Background:Catalytically inactive regulatory factor of DNA methyltransferases that can either promote or inhibit DNA methylation depending on the context (PubMed:11719692, PubMed:15318244, PubMed:15671018, PubMed:24074865). Essential for the function of DNMT3A and DNMT3B: activates DNMT3A and DNMT3B by binding to their catalytic domain (PubMed:15671018). Acts by accelerating the binding of DNA and S-adenosyl-L-methionine (AdoMet) to the methyltransferases and dissociates from the complex after DNA binding to the methyltransferases (PubMed:15671018). Recognizes unmethylated histone H3 lysine 4 (H3K4me0) and induces de novo DNA methylation by recruitment or activation of DNMT3 (By similarity). Plays a key role in embryonic stem cells and germ cells (PubMed:11719692, PubMed:15318244, PubMed:24074865). In germ cells, required for the methylation of imprinted loci together with DNMT3A (PubMed:11719692). In male germ cells, specifically required to methylate retrotransposons, preventing their mobilization (PubMed:15318244). Plays a key role in embryonic stem cells (ESCs) by acting both as an positive and negative regulator of DNA methylation (PubMed:24074865). While it promotes DNA methylation of housekeeping genes together with DNMT3A and DNMT3B, it also acts as an inhibitor of DNA methylation at the promoter of bivalent genes (PubMed:24074865). Interacts with the EZH2 component of the PRC2/EED-EZH2 complex, preventing interaction of DNMT3A and DNMT3B with the PRC2/EED-EZH2 complex, leading to maintain low methylation levels at the promoters of bivalent genes (PubMed:24074865). Promotes differentiation of ESCs into primordial germ cells by inhibiting DNA methylation at the promoter of RHOX5, thereby activating its expression (PubMed:24074865). {ECO:0000250|UniProtKB:Q9UJW3, ECO:0000269|PubMed:11719692, ECO:0000269|PubMed:15318244, ECO:0000269|PubMed:15671018, ECO:0000269|PubMed:24074865}.

Research_area:

Applications:ELISA

Target_protein:Dnmt3l

Reactivity:Mouse

Assay type:Sandwich

Sensitivity:0.1ng/ml

Detection range:0.19-12ng/ml

Sample type:Serum, plasma or other biological fluids

Full product name:DNA

Species:Mouse

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:Q9CWR8

MASS(Da):47993

GeneID:54427

Synonyms:Dnmt3l

Gene names:Dnmt3l

Standard solution:24ng/ml