Mouse CYR61(Cysteine Rich Protein, Angiogenic Inducer 61) Microsample ELISA Kit

SKU: ELK10781MS

Size:96T

Reactivity:Mouse

Alternative Names:CCN1; GIG1; IGFBP10; CCN family member 1; Insulin-like growth factor-binding protein 10

Assay Type:Sandwich

Sensitivity:11.5 pg/mL

Standard:2000 pg/mL

Detection range:31.25-2000 pg/mL

Sample type:serum, plasma, tissue homogenates and other biological fluids

Assay length:3.5h

Research Area:Tumor immunity;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CYR61. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CYR61. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CYR61, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CYR61 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:P18406