Mouse Caspase 4,Casp4 ELISA KIT

SKU: E0943Mo

Size:96 Tests-1 Kit

Background:Inflammatory caspase that acts as an essential effector of NLRP3 inflammasome-dependent CASP1 activation and IL1B and IL18 secretion in response to non-canonical activators, such as UVB radiation, cholera enterotoxin subunit B and cytosolic LPS, as well as infection with Gram-negative bacteria (PubMed:8702803, PubMed:9038361, PubMed:22002608, PubMed:25119034). Thiol protease that cleaves a tetrapeptide after an Asp residue at position P1 (By similarity). Independently of NLRP3 inflammasome and CASP1, promotes pyroptosis, through GSDMD cleavage and activation, followed by IL1A, IL18 and HMGB1 release in response to non-canonical inflammasome activators (PubMed:22002608, PubMed:26320999, PubMed:26375003). Plays a crucial role in the restriction of Salmonella typhimurium replication in colonic epithelial cells during infection: in later stages of the infection, LPS from cytosolic Salmonella triggers CASP4 activation, which catalyzes cleavage of GSDMD, resulting in pyroptosis of infected cells and their extrusion into the gut lumen, as well as in IL18 secretion (PubMed:25121752, PubMed:26375003). Cleavage of GSDMD is not strictly dependent on the consensus cleavage site but depends on an exosite interface on CASP4 that recognizes and binds the Gasdermin-D, C-terminal (GSDMD-CT) part (PubMed:32554464, PubMed:32109412). Pyroptosis limits bacterial replication, while cytokine secretion promotes the recruitment and activation of immune cells and triggers mucosal inflammation (PubMed:25121752). Involved in LPS-induced IL6 secretion; this activity may not require caspase enzymatic activity (By similarity). Involved in cell death induced by endoplasmic reticulum stress (By similarity). Activated by direct binding to LPS without the need of an upstream sensor (PubMed:25119034). Does not directly process IL1B (PubMed:8702803, PubMed:9038361). During non-canonical inflammasome activation, cuts CGAS and may play a role in the regulation of antiviral innate immune activation (PubMed:28314590). {ECO:0000250|UniProtKB:P49662, ECO:0000269|PubMed:22002608, ECO:0000269|PubMed:25119034, ECO:0000269|PubMed:25121752, ECO:0000269|PubMed:26320999, ECO:0000269|PubMed:26375003, ECO:0000269|PubMed:28314590, ECO:0000269|PubMed:32109412, ECO:0000269|PubMed:32554464, ECO:0000269|PubMed:8702803, ECO:0000269|PubMed:9038361}.

Research_area:

Applications:ELISA

Target_protein:Casp4

Reactivity:Mouse

Assay type:Sandwich

Sensitivity:1.82ng/ml

Detection range:3.75-240ng/ml

Sample type:Serum, plasma or other biological fluids

Full product name:Caspase-4

Species:Mouse

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:P70343

MASS(Da):42742

GeneID:12363

Synonyms:Casp4 Casp11 Caspl Ich3

Gene names:Casp4

Standard solution:480ng/ml