IL17 ELISA kit (Chicken)

SKU: CEA063Ga

Size: 96Tests

# of Times Cited in literature: 1

Prepare Time: 2-7 days(please inquire for mutiple units)

Target Name: IL17

Target Full Name: Interleukin 17

Alternative Names: IL17A; CTLA8; IL-17A; Cytotoxic T-Lymphocyte-Associated Protein 8

Target Species: Chicken

Uniprot: Q7T1P7

Gene ID: 395111

Featured Series: CE kit

Featured Series Function: Detects small molecule

Specificity: Reactive with Chicken IL17 / Interleukin 17

Method: Colormetric

Detection principle: Competitive Inhibition

Detection range: 62.5-1,000pg/mL

Sensitivity: 26.6pg/mL

Assay Time: 2h

Sample Size: 50uL

Recommended/Predicted Sample Types: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 17 (IL17) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 17 (IL17) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Interleukin 17 (IL17). No significant cross-reactivity or interference between Interleukin 17 (IL17) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C; 3. Aspirate and wash 3 times; 4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 5. Aspirate and wash 5 times; 6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 7. Add 50µL Stop Solution. Read at 450 nm immediately.

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Interleukin 17 (IL17) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Interleukin 17 (IL17) and unlabeled Interleukin 17 (IL17) (Standards or samples) with the pre-coated antibody specific to Interleukin 17 (IL17). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Interleukin 17 (IL17) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Interleukin 17 (IL17) in the sample.

Research Area: Cytokine;Infection immunity;

References Citing This Product: Characterization of Simian Immunodeficiency Virus Variants Anatomically Compartmentalized inPlasma and Milk in Chronically Infected African Green Monkeys.