IHOG Monoclonal Antibody

SKU: BT-MCA2193

Size:100μL

Background:The ihog gene (interference hedgehog), identified by RNA interference in Drosophila cultured cells, encodes a type 1 membrane protein shown here to bind and to mediate response to the active Hedgehog (Hh) protein signal. ihog mutations produce defects characteristic of Hh signaling loss in embryos and imaginal discs, and epistasis analysis places ihog action at or upstream of the negatively acting receptor component, Patched (Ptc). The first of two extracellular fibronectin type III (FNIII) domains of the Ihog protein mediates a specific interaction with Hh protein in vitro, but the second FNIII domain is additionally required for in vivo signaling activity and for Ihog-enhanced binding of Hh protein to cells coexpressing Ptc. Other members of the Ihog family, including Drosophila Boi and mammalian CDO and BOC, also interact with Hh ligands via a specific FNIII domain, thus identifying an evolutionarily conserved family of membrane proteins that function in Hh signal response.

Research_area:Others

Target_protein:IHOG

applications:

Reactivity:Drosophila Melanogaster

Clonality:Monoclonal

Clone ID:3G8

Host:Mouse

Isotype:Mouse IgG1

Gene Symbol/ Name:IHOG

Immunogen:Purified recombinant fragment of human IHOG expressed in E. Coli.

Storage:4°C|-20°C for long term storage

Purification:

Formulation:Ascitic fluid containing 0.03% sodium azide.

Concentration:

Molecular weight(Da):98kDa

UniProt accession:Q9VM64

Synonyms:CG9211, CT26314, Dmel CG9211, ihog, Ihog

GeneID:33972